The development of long-read transcriptome sequencing (LR-RNAseq) promises to facilitate the process of genome annotation. By providing reads spanning repeats and direct exon/exon connectivity, LR-RNAseq represents an unfragmented vision of the transcriptome which thus allows the refinement of gene/transcript reconstruction and quantification. Yet, it requires the development of novel bioinformatic solutions specifically adapted to these novel technologies.
In this presentation, we will briefly introduce the advantages of long-read sequencing technologies for both DNA and RNA analyses, then I’ll present the long-read sequencing platform IGDRion (https://igdr.univ-rennes.fr/igdrion) that we developed in Rennes and finally, I’ll illustrate the interest of LR-RNAseq in order to ease the annotation of long non-coding RNAs (lncRNAs).
Symbiose groups seminars : https://www.cesgo.org/symbiose/seminars/nanopore-long-read-sequencing-a…