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Jeudi 2 Décembre, Thomas Derrien (Institut de Génétique et Développement de Rennes 1) Print
Written by Pierre PETERLONGO   

Characterization of Long non coding RNAs in the ENCODE project using NGS sequencing

10h30 Salle Aurigny

Deciphering the function of the ever-growing class of long non-coding RNAs (LnRNAs) is one of the remaining challenges of biology.  To identify LnRNAs in humans and to assign a function to them, we interrogated the transcripts annotated by the ENCODE project, excluding protein-coding genes and their promoters.  This analysis revealed 3019 annotated non-coding transcripts with defined exon-intron structure and an average size of about 800 base pairs.  These transcripts display evolutionary sequence conservation and a specific pattern of splicing distinct from that of protein-coding transcripts.  Moreover, using high throughput RNA sequencing data (RNA_Seq), we refined the measurements of their expression levels and show that they display differential tissue expression, which is closely paralleled with their associated active or repressive chromatin signatures. Unexpectedly, functional analysis of selected cases of LnRNAs in multiple cell lines revealed their positive regulation of the neighboring protein-coding transcripts, including the master regulator of hematopoiesis, stem cell leukemia gene (SCL).  These results reveal an unanticipated role for LnRNAs in regulation of transcription of the neighboring protein-coding genes.
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